Core Facility Microbiome

The Microbiome Core Facility is dedicated to researching the complex links between intestinal microbiota and their host organisms. The Next Generation Sequencing (NGS) enables the scientists to carry out complete analyses of the intestinal bacterial community (microbiome). The Core Facility team has expert knowledge in the areas of microbiology, microbial ecology, bacterial genetics, high-throughput sequencing and bioinformatics.

Core Facility Microbiome

Picture: Allan Richard Tobis

The Microbiome Core Facility provides a modern research platform in the area of high-throughput sequencing. Its expertise ranges from the identification of individual bacterial strains and the composition of a complex bacterial community to targeted studies on the functionality of individual bacteria and communities in a host organism or other habitats.

Bacterial diversity and composition can be determined very accurately in biological samples using sequencing. The 16S rRNA gene is usually used as a basis here. Through the determination of the base sequence from a sample, it is possible to extrapolate the composition of the microbial communities. The data is generated using Illumina technology and analyzed using self-developed bioinformatics software (https://www.imngs.org/). The composition of the microbiota can then be examined further from functional perspectives. This enables the researchers to determine, for example, whether the presence or absence of certain bacteria associated with functional gains or losses or whether certain metabolites generated.

Other methods, such as the sequencing of individual bacterial genomes, the sequencing of the transcripts (RNAseq) and the sequencing of the actively translated mRNAs (RIBOseq) are also available for the further identification of regulatory pathways and active genes.